27IV

Crystal structure of the mature form of human ASPRV1 bound to self-cleavage peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	193
Detector technology	PIXEL
Collection date	2026-01-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	50.130, 61.265, 76.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.380 - 1.950
R-factor	0.1963
Rwork	0.191
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.838
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.770	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	17760	1241
<I/σ(I)>	22.3
Completeness [%]	100.0
Redundancy	12.6
CC(1/2)	1.000	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M calcium chloride dihydrate, 0.1 M HEPES sodium, pH 7.5, and 28% (v/v) PEG 400