27FM

Crystal structure of human glyceraldehyde-3-phosphate dehydrogenase bound to gold(I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97914
Spacegroup name	P 1 21 1
Unit cell lengths	80.880, 128.590, 81.750
Unit cell angles	90.00, 117.98, 90.00

Refinement procedure

Resolution	64.300 - 1.460
R-factor	0.1515
Rwork	0.151
R-free	0.16670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.827
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.300	1.480
High resolution limit [Å]	1.460	1.460
Rmerge	0.074	0.854
Number of reflections	254607	8464
<I/σ(I)>	10.07
Completeness [%]	99.8
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15 M D,L-malic acid, 20% PEG 3350 (w/v)