279D
CRYSTAL STRUCTURE OF THE SELF-COMPLEMENTARY 5'-PURINE START DECAMER D(GCGCGCGCGC) IN THE Z-DNA CONFORMATION-PART I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-04-13 |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 18.100, 18.100, 43.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 31.000 * |
| Data reduction software | XENGEN (V. 2.0) |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.022 |
| Total number of observations | 2020 * |
| Number of reflections | 401 |
| Completeness [%] | 96.4 |
| Redundancy | 5.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | decamer | 1 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | magnesium chloride | 5 (mM) | |
| 4 | 1 | drop | spermine tetrachloride | 0.5 (mM) | |
| 5 | 1 | drop | MPD | 5 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 45 (%) |
