26UI

Dimeric C-terminal domain of Nucleocapsid protein of SARS-CoV-2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2025-02-03
Detector	RAYONIX MX-225
Wavelength(s)	0.97777
Spacegroup name	P 1 21 1
Unit cell lengths	43.463, 43.527, 54.969
Unit cell angles	90.00, 107.58, 90.00

Refinement procedure

Resolution	30.010 - 1.850
R-factor	0.1721
Rwork	0.170
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.781
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.530	1.890
High resolution limit [Å]	1.850	1.850
Rmeas		0.915
Number of reflections	30272	875
<I/σ(I)>	10
Completeness [%]	98.2	83.7
Redundancy	3.6
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	294	0.2M ammonium chloride, 0.1M sodium acetate pH 5, 20% PEG 6000