26TP

Crystal structure of the Pseudomonas aeruginosa MlaC-MlaD complex in space group P1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	61.713, 88.639, 88.814
Unit cell angles	64.65, 72.37, 72.28

Refinement procedure

Resolution	47.440 - 2.560
R-factor	0.2514
Rwork	0.249
R-free	0.30280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.558
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.560	2.560
Rmerge	0.317	1.178
Rmeas	0.393	1.475
Number of reflections	99657	15794
<I/σ(I)>	3.1	0.79
Completeness [%]	97.0	95.5
Redundancy	2.6
CC(1/2)	0.878	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium citrate (pH 5.0), 29% Jeffamine ED-2001 (pH 7.0)