26RV
E coli BFR variant 4QY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-04-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 115.520, 115.520, 115.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.510 - 1.890 |
| R-factor | 0.2176 |
| Rwork | 0.215 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.353 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.160 | 1.940 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 39645 | 1565 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 36.1 | |
| CC(1/2) | 0.999 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 2.5M NaCl 100 mM MgCl2 |
