26RC
Crystal Structure of N-terminal C1 domain of KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.449, 107.449, 218.762 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.150 - 2.880 |
| R-factor | 0.2404 |
| Rwork | 0.238 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.773 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.1_6048) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 2.890 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.140 | 0.908 |
| Number of reflections | 37112 | 3578 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.5 | |
| Redundancy | 10.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 303 | 100mM Tris-HCl, 130-160mM MgSO4, 31-34% (w/v) PEG 400, 5mM adenylyl imidodiphosphate (AMP-PNP) |
