26QZ

Crystal structure of Rhodostomin ARGDP mutant

Replaces:  4M4C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-16
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97622
Spacegroup name	P 1
Unit cell lengths	31.619, 42.637, 46.538
Unit cell angles	106.84, 103.86, 97.23

Refinement procedure

Resolution	23.581 - 1.804
Rwork	0.157
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.476
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.059	0.459
Number of reflections	19633	1886
<I/σ(I)>	23.9
Completeness [%]	96.7
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG4000, 0.2M Ammonium sulfate, 5% PEG3350, 2% PEG200, 0.5% 2-propanol(External) , pH 8.0