26EE
Rhodobacter sp. 140A Polyphosphate kinase mutant-D114K/D210S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-03-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.300, 65.450, 90.360 |
| Unit cell angles | 104.50, 101.04, 100.82 |
Refinement procedure
| Resolution | 61.459 - 2.430 |
| Rwork | 0.220 |
| R-free | 0.26690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.054 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.459 | 2.490 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.065 | 0.790 |
| Number of reflections | 44219 | 3248 |
| <I/σ(I)> | 11.9 | 1.7 |
| Completeness [%] | 95.5 | 95.4 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.996 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M MgCl2, 0.1 M Tris-HCl pH 8.5, 25% (w/v) PEG 3350 |
