26BH
Crystal structure of a Mrr domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.155, 103.433, 88.723 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 44.680 - 2.100 |
| R-factor | 0.1919 |
| Rwork | 0.188 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.879 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.680 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 36974 | 7883 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 92.2 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.991 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M BICINE pH 8.5 20% w/v Polyethylene glycol 10,000 |
