264D
THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR-GROOVE-BINDING DRUG HOECHST 33258
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.500, 41.400, 65.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 * |
| R-factor | 0.2 |
| Rwork | 0.200 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.710 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 5.000 * |
| High resolution limit [Å] | 2.400 * |
| Rmerge | 0.061 * |
| Total number of observations | 9285 * |
| Number of reflections | 2291 |
| Completeness [%] | 82.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA dodecamer | 1 (mM) | |
| 2 | 1 | drop | Hoechst-33258 | 1 (mM) | |
| 3 | 1 | drop | MPD | 19.5 (%) | |
| 4 | 1 | drop | sodium cacodylate | 1 (mM) | |
| 5 | 1 | drop | spermine | 0.7 (mM) | |
| 6 | 1 | reservoir | 50 (%) |
