261D
CRYSTAL STRUCTURE OF THE DNA DECAMER D(CGCAATTGCG) COMPLEXED WITH THE MINOR GROOVE BINDING DRUG NETROPSIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-10-12 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.840, 39.800, 54.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.800 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.030 |
| Number of reflections | 1864 |
| Completeness [%] | 82.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 291.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | NA CACODYLATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.83 (mM) | |
| 2 | 1 | drop | netropsin | 1.7 (mM) | |
| 3 | 1 | drop | 0.17 (M) | ||
| 4 | 1 | drop | MPD | 11 (%) | |
| 5 | 1 | reservoir | 45 (%) |
