260L

AN ADAPTABLE METAL-BINDING SITE ENGINEERED INTO T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1998-01-19
Detector	SDMS
Spacegroup name	P 32 2 1
Unit cell lengths	62.170, 62.170, 96.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.800
Rwork	0.190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	2.160
Data reduction software	SDMS (DETECTOR SYSTEM (NIELSEN))
Data scaling software	SDMS (DETECTOR SYSTEM (NIELSEN))
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.031	0.180
Number of reflections	19963
<I/σ(I)>		2
Completeness [%]	92.0
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	HEPES
10	1	2	NICL2
2	1	1	NACL
3	1	1	ISOPROPANOL
4	1	1	PEG 8000
5	1	1	NICL2
6	1	2	HEPES
7	1	2	NACL
8	1	2	ISOPROPANOL
9	1	2	PEG 8000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		200-300 (mM)
2	1	reservoir	HEPES	20 (mM)
3	1	reservoir	PEG8000	12-16 (%)
4	1	reservoir	2-propanol	10 (%)
5	1	reservoir	metal ion	2 (mM)	as the chloride or acetate salt
6	1	drop	protein	1 (mM)
7	1	drop	HEPES	20 (mM)