25WN

Crystal structure of Candida albicans Eukaryotic translation initiation factor 5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-03-05
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 1 21 1
Unit cell lengths	35.253, 35.149, 337.128
Unit cell angles	90.00, 93.00, 90.00

Refinement procedure

Resolution	19.390 - 2.600
Rwork	0.333
R-free	0.34840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	3.102
Data scaling software	Aimless (0.8.2)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.128))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.390	19.390	2.720
High resolution limit [Å]	2.600	9.010	2.600
Rmerge	0.288	0.095	1.345
Rmeas	0.388	0.134	1.798
Rpim	0.259	0.095	1.185
Number of reflections	24965	503	3093
<I/σ(I)>	4.6	6.7	1.3
Completeness [%]	96.0	75.8	95.6
Redundancy	3.1	2.2	3.2
CC(1/2)	0.910	0.964	0.277

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	5% PEG 3350, 200mM sodium formiate, 10% glycerol, 50mM MES pH 6