25RT
Crystal Structure of IphB from Comamonas testosteroni KF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.030, 75.700, 73.640 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 72.058 - 1.518 |
| Rwork | 0.132 |
| R-free | 0.16180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.791 |
| Data reduction software | AutoProcess |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0431 (refmacat 0.4.126)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.060 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Number of reflections | 71252 | 3522 |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.990 | 0.763 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium sulfate, 0.1M sodium cacodylate trihydrate pH 6.5, 30% PEG8000 |
