25MN
Lysine racemase TM1597
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.907, 138.474, 174.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.920 - 2.150 |
| R-factor | 0.17986 |
| Rwork | 0.177 |
| R-free | 0.23968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Colabfold |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.341 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.154 | 1.252 |
| Rmeas | 0.167 | 1.354 |
| Rpim | 0.064 | 0.512 |
| Number of reflections | 119042 | 5864 |
| <I/σ(I)> | 8.9 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.995 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 303.15 | 10% PEG 6000, 0.8 M LiCl, and 0.1 M MES-NaOH (pH 6.0) |
