259L
AN ADAPTABLE METAL-BINDING SITE ENGINEERED INTO T4 LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-03-16 |
| Detector | SDMS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 62.140, 62.140, 96.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.920 |
| Rwork | 0.186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.300 |
| Data reduction software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Data scaling software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.031 | 0.200 |
| Number of reflections | 15465 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 87.0 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | ISOPROPANOL | ||
| 4 | 1 | 1 | PEG 8000 | ||
| 5 | 1 | 2 | HEPES | ||
| 6 | 1 | 2 | NACL | ||
| 7 | 1 | 2 | ISOPROPANOL | ||
| 8 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 200-300 (mM) | ||
| 2 | 1 | reservoir | HEPES | 20 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 12-16 (%) | |
| 4 | 1 | reservoir | 2-propanol | 10 (%) | |
| 5 | 1 | reservoir | metal ion | 2 (mM) | as the chloride or acetate salt |
| 6 | 1 | drop | protein | 1 (mM) | |
| 7 | 1 | drop | HEPES | 20 (mM) |
