259D
RNA HYDRATION: A DETAILED LOOK
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-23 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 42.260, 42.260, 130.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.460 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.26300 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 3.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | |
| High resolution limit [Å] | 1.460 | 1.460 * |
| Rmerge | 0.051 | 0.499 * |
| Number of reflections | 8040 | |
| Completeness [%] | 99.6 | 97.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | pH 4.60, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA ACETATE | ||
| 3 | 1 | 1 | NH4 SULFATE | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | NA ACETATE | ||
| 6 | 1 | 2 | NH4 SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA oligonucleotide | 1 (mM) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 1 (M) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2 (M) |
