258L
AN ADAPTABLE METAL-BINDING SITE ENGINEERED INTO T4 LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-12-09 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.330, 61.330, 97.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.300 |
| Data reduction software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Data scaling software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.032 | 0.180 |
| Number of reflections | 17343 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 82.0 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 200-300 (mM) | ||
| 2 | 1 | reservoir | HEPES | 20 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 12-16 (%) | |
| 4 | 1 | reservoir | 2-propanol | 10 (%) | |
| 5 | 1 | reservoir | metal ion | 2 (mM) | as the chloride or acetate salt |
| 6 | 1 | drop | protein | 1 (mM) | |
| 7 | 1 | drop | HEPES | 20 (mM) |
