257L

AN ADAPTABLE METAL-BINDING SITE ENGINEERED INTO T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1998-01-24
Detector	SDMS
Spacegroup name	P 32 2 1
Unit cell lengths	60.930, 60.930, 96.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
Rwork	0.174
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	2.500
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.033	0.200
Number of reflections	15507
<I/σ(I)>		2
Completeness [%]	89.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	4 *	CRYSTALS GROWN IN HANGING DROPS AT 4 DEGREES C. PROTEIN 10-20 MG/ML IN A BUFFER CONTAINING SODIUM PHOSPHATE PH 5.4, 200 MM NACL, WAS DILUTED 1/2 WITH A WELL SOLUTION CONTAINING 1.8-2.0 M NA/K PHOSPHATE, 200MM NACL, PH 6.5-7.5, AND 5MM OXIDIZED BME 10% V/V ISOPROPANOL,18% W/V PEG 8000, PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium potassium phosphate	1.8-2.0 (M)
2	1	reservoir	oxidized BME	5 (mM)
3	1	drop	protein	1 (mM)
4	1	drop		200 (mM)
5	1	drop	phosphate	100 (mM)