255D
CRYSTAL STRUCTURE OF AN RNA DOUBLE HELIX INCORPORATING A TRACK OF NON-WATSON-CRICK BASE PAIRS
Experimental procedure
| Source type | ROTATING ANODE |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 38.280, 32.280, 36.380 |
| Unit cell angles | 90.00, 124.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.164 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 2280 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | pH 8.50, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | BIS-TRIS-PROPANE | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | NA CITRATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | PEG 400 | ||
| 8 | 1 | 2 | NA CITRATE | ||
| 9 | 1 | 2 | MGCL2 |
