253D
CRYSTAL STRUCTURE OF THE B-DNA NONAMER D(GCGTACGCG) WITH A NOVEL D[G*(G.C)] BASE-TRIPLET INVOLVING THE MINOR GROOVE
Experimental procedure
| Source type | SEALED TUBE |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1995-08-20 |
| Detector | SIEMENS P4 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 38.915, 38.915, 33.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.1931 |
| Rwork | 0.193 |
| R-free | 0.21370 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.620 |
| Data reduction software | XSCANS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.042 |
| Number of reflections | 1126 |
| Completeness [%] | 76.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
