251D
SINGLE CRYSTAL AND MOLECULAR STRUCTURE OF D(CTCGAG)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-06-10 |
| Detector | SIEMENS |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 40.140, 40.140, 44.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.900 |
| Data reduction software | XENGEN (V. 2.0) |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 * |
| Rmerge | 0.050 |
| Number of reflections | 1547 |
| Completeness [%] | 83.0 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 1 | NA CACODYLATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 1 (mM) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | spermine tetrahydrochloride | 1 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 5 | 1 | drop | MPD | 5 (%) | |
| 6 | 1 | reservoir | MPD | 40 (%) |
