24XP
Crystal structure of WDR5 F133A variant in complex with MBD3C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.806, 46.928, 102.953 |
| Unit cell angles | 90.00, 107.33, 90.00 |
Refinement procedure
| Resolution | 30.930 - 1.300 |
| R-factor | 0.1726 |
| Rwork | 0.172 |
| R-free | 0.17990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.992 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.280 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.082 | 0.701 |
| Rmeas | 0.089 | 0.761 |
| Rpim | 0.035 | 0.293 |
| Total number of observations | 138991 | |
| Number of reflections | 143721 | 21006 |
| <I/σ(I)> | 11.4 | 2.4 |
| Completeness [%] | 98.9 | |
| Redundancy | 6.6 | 6.6 |
| CC(1/2) | 0.998 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M sodium acetate (pH 5.0), 20% (w/v) PEG 4000 |
