24UY

Crystal structure of FPP-methyltransferase PcFPPMT from Pseudomonas chlororaphis O6 in complex with SAH and GPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 07A
Synchrotron site	NSRRC
Beamline	TPS 07A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-26
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97624
Spacegroup name	P 21 21 2
Unit cell lengths	68.686, 98.353, 42.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.160 - 1.700
R-factor	0.1957
Rwork	0.194
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.003
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.039	0.028	0.479
Rmeas	0.044	0.032	0.549
Rpim	0.020	0.015	0.262
Total number of observations	158970
Number of reflections	31615	3292	3072
<I/σ(I)>	16.7
Completeness [%]	98.4	95.7	97
Redundancy	5	4.5	4
CC(1/2)	0.996	0.998	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	25% PEG 3350, 0.2 M MgCl2, 0.1 M Imidazole, pH 7.5