24UX

Crystal structure of FPP-methyltransferase PcFPPMT from Pseudomonas chlororaphis O6 in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 07A
Synchrotron site	NSRRC
Beamline	TPS 07A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-26
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97624
Spacegroup name	P 21 21 2
Unit cell lengths	68.533, 98.147, 42.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.200 - 1.200
R-factor	0.1952
Rwork	0.194
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.888
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.240
High resolution limit [Å]	1.200	2.580	1.200
Rmerge	0.051	0.044	0.501
Rmeas	0.058	0.050	0.571
Rpim	0.026	0.022	0.267
Total number of observations	463882
Number of reflections	88891	9074	8709
<I/σ(I)>	12.4
Completeness [%]	99.2	96.5	98.5
Redundancy	5.2	4.7	4.2
CC(1/2)	0.992	0.997	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	25% PEG 3350, 0.2 M MgCl2, 0.1 M Imidazole, pH 7.5