24UU

Crystal structure of Endonuclease IV from Chlamydophila pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-07
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	67.930, 68.080, 150.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.030 - 1.970
Rwork	0.178
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.812
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.050	2.020
High resolution limit [Å]	1.970	1.970
Rmerge	0.073	1.263
Number of reflections	50308	3474
<I/σ(I)>	18.3	2
Completeness [%]	100.0	100
Redundancy	12.7	10.2
CC(1/2)	0.999	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	Precipitant: 8% v/v Tacsimate pH 5.2,25% w/v PEG 3350.Protein: 2.81 mg/mL protein, 100mmol/L NaCl, 20mmol/L Tris-HCl pH7.8. Mix the precipitant with the protein solution at a ratio of 1:1.