24UI
Cu(II)-bound SLA1 dimer (H67/H71/C96)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.11584 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.792, 51.798, 48.749 |
| Unit cell angles | 90.00, 97.02, 90.00 |
Refinement procedure
| Resolution | 43.670 - 2.160 |
| R-factor | 0.2401 |
| Rwork | 0.231 |
| R-free | 0.32190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.195 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.670 | 2.237 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.041 | 0.575 |
| Rmeas | 0.058 | 0.815 |
| Rpim | 0.041 | 0.576 |
| Number of reflections | 10940 | 1077 |
| <I/σ(I)> | 11.49 | 1.33 |
| Completeness [%] | 99.5 | 99.72 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.996 | 0.494 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 24% PEG 1500, 200mM (NH4)2SO4, 100mM CHES, pH 9 |
