24UG
Human CLIC1 mutant-Y214A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-02-09 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.944, 68.388, 81.751 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 22.870 - 2.500 |
| R-factor | 0.1878 |
| Rwork | 0.182 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fbq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.571 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER (1.21.2_5419) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.250 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.123 | 0.247 |
| Rpim | 0.040 | |
| Number of reflections | 16105 | 1813 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.4 | 9.4 |
| CC(1/2) | 0.995 | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | 0.2 M Magnesium chloride hexahydrate, 20% PEG 6000, 100 mM MES pH 6.0 |
