24TG
Crystal structure of selenomethionine labelled Streptomyces avermitilis endo-beta-1,6-galactanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.173, 233.013, 87.885 |
| Unit cell angles | 90.00, 94.86, 90.00 |
Refinement procedure
| Resolution | 43.769 - 2.001 |
| Rwork | 0.169 |
| R-free | 0.21480 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.291 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.149 | 0.392 |
| Number of reflections | 114201 | 11437 |
| <I/σ(I)> | 14.4 | 4.8 |
| Completeness [%] | 98.4 | |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 17% PEG4000, 20% isopropanol, 0.1 M Sodium citrate pH 5.6 |
