24SU

Structure of Cacipacore virus helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	64.560, 99.860, 158.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.870 - 2.070
R-factor	0.1907
Rwork	0.188
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.882
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.870	2.140
High resolution limit [Å]	2.070	2.070
Number of reflections	31630	4112
<I/σ(I)>	15.58
Completeness [%]	99.8	99.8
Redundancy	11.3
CC(1/2)	0.997	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEGRx1-2