24PV

The crystal structure of the chicken MHF1-MHF2(L77C) disulfide-crosslinked complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	80.794, 86.583, 221.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.290 - 4.000
R-factor	0.2621
Rwork	0.253
R-free	0.33770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.633
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.290	4.143
High resolution limit [Å]	4.000	4.000
Rmerge	0.138	0.483
Rmeas	0.150	0.521
Rpim	0.058	0.195
Number of reflections	12579	663
<I/σ(I)>	10.35	4.38
Completeness [%]	99.3	100
Redundancy	6.6	7
CC(1/2)	0.995	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.7	293	0.1M Tris Bicine pH 8.8, 100mM Carboxylic acid mix, 14% MPD, 20.5% PEG 3350, 250mM NaCl