24NK
Crystal Structure of Cypridina Luciferase complexed with Oxyluciferin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 144.648, 144.648, 186.154 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.350 - 3.140 |
| R-factor | 0.2685 |
| Rwork | 0.266 |
| R-free | 0.32481 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.889 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.350 | 3.360 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Rmerge | 0.221 | 1.679 |
| Rmeas | 0.233 | 1.762 |
| Rpim | 0.071 | 0.533 |
| Number of reflections | 20708 | 3653 |
| <I/σ(I)> | 14 | 2.6 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 19.5 | 20.5 |
| CC(1/2) | 0.999 | 0.981 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 7 | 293 | 25mg/mL CLuc complexed with oxyluciferin, 3% polyethylene glycol monomethyl ether 2000, 7% polypropylene glycol 400, 2M ammonium phosphate |
