24NE
Crystal Structure of Cypridina luciferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-18 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 2.70001 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 144.370, 144.370, 186.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.300 - 3.000 |
| R-factor | 0.23488 |
| Rwork | 0.233 |
| R-free | 0.26841 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.462 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.205 | 2.878 |
| Number of reflections | 53534 | 8599 |
| <I/σ(I)> | 48.94 | 2.73 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 294 | 152 |
| CC(1/2) | 1.000 | 0.983 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 7 | 293 | 25mg/mL CLuc, 3% polyethylene glycol monomethyl ether 2000, 7% polypropylene glycol 400, 2M ammonium phosphate |
