24KU

Crystal structure of the Aeropyrum pernix PCNA1 monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-13
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.00000
Spacegroup name	P 43 21 2
Unit cell lengths	68.927, 68.927, 120.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.630 - 1.600
R-factor	0.1989
Rwork	0.198
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.882
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.300	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.067	0.368
Rmeas	0.074	0.429
Rpim	0.031	0.195
Number of reflections	72556	3706
<I/σ(I)>	20.1	3.4
Completeness [%]	99.8	97.7
Redundancy	10.6	7.8
CC(1/2)	0.998	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.1 M citrate (pH 4.5), 1.8 M ammonium sulfate