24JS

Crystal structure of voltage-gated sodium channel NavAb N49K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NanoTerasu BEAMLINE BL09U MX-ES
Synchrotron site	NanoTerasu
Beamline	BL09U MX-ES
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-03-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	I 4 2 2
Unit cell lengths	126.850, 126.850, 203.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.360 - 3.500
R-factor	0.2532
Rwork	0.252
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.703
Data reduction software	XDS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.910	3.625
High resolution limit [Å]	3.500	3.500
Rpim	0.071
Number of reflections	10990	803
<I/σ(I)>	21.15	3.11
Completeness [%]	96.7
Redundancy	90.9
CC(1/2)	0.983	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4