24FO

Artificial Copper-binding trimeric protein 1 (Cu(II)TP6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-10
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 3
Unit cell lengths	115.865, 115.865, 39.128
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.970 - 2.100
R-factor	0.2052
Rwork	0.203
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.520
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.970	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	34493	34493
<I/σ(I)>	9.88
Completeness [%]	99.9
Redundancy	10.5
CC(1/2)	0.977	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M Tris-HCl (pH 8.5) buffer containing 0.2 M trimethylamine N-oxide and 15% (w/v) PEG 2000 MME