24CA

Crystal structure of nuclease MYG1(D93A) bound to Mn2+ and AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2026-01-25
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	70.840, 97.159, 116.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.240 - 1.930
R-factor	0.1786
Rwork	0.177
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.814
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.240	1.990
High resolution limit [Å]	1.930	1.930
Number of reflections	30324	4415
<I/σ(I)>	16
Completeness [%]	98.6
Redundancy	13.1
CC(1/2)	0.990	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.15 M ammonium sulfate, 0.1 M Hepes, pH 7.0, 20% w/v PEG 4000