24AW

Crystal structure of nuclease MYG1 bound to Mn2+ and dCMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	70.320, 97.730, 116.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.660 - 1.860
R-factor	0.1761
Rwork	0.175
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.802
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.660	1.920
High resolution limit [Å]	1.860	1.860
Number of reflections	33733	2220
<I/σ(I)>	15.6
Completeness [%]	98.9
Redundancy	10.4
CC(1/2)	0.990	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Hepes, pH 7.0, 15% w/v PEG 20000