24AP

Crystal structure of nuclease MYG1 bound to Mn2+ and UMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-11-03
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	70.220, 98.560, 116.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.490 - 1.780
R-factor	0.2044
Rwork	0.203
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.781
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.490	1.840
High resolution limit [Å]	1.780	1.780
Number of reflections	37494	5171
<I/σ(I)>	12
Completeness [%]	95.4
Redundancy	6.2
CC(1/2)	0.990	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5, 15% w/v PEG 6000