24AI

alpha-1,2-glucosidase from Arthrobacter humicola A8F5, kojibiose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	55.784, 109.457, 105.372
Unit cell angles	90.00, 93.27, 90.00

Refinement procedure

Resolution	48.600 - 1.790
R-factor	0.17045
Rwork	0.168
R-free	0.20992
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.015
RMSD bond angle	2.221
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.640	1.900
High resolution limit [Å]	1.790	1.790
Rmerge	0.226	1.495
Rmeas	0.237	1.571
Number of reflections	119031	18995
<I/σ(I)>	14.21	2.36
Completeness [%]	99.8	99.1
Redundancy	11.4	10.7
CC(1/2)	0.997	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	0.1 M Sodium acetate pH5.1, 34%(v/v)1,2-propanediol