24AH

Crystal structure of nuclease MYG1 bound to Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-11-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	70.086, 98.507, 117.174
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.590 - 2.580
R-factor	0.2093
Rwork	0.207
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.961
Data reduction software	HKL-2000 (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.590	2.670
High resolution limit [Å]	2.580	2.580
Number of reflections	13058	1868
<I/σ(I)>	8.1
Completeness [%]	99.8
Redundancy	6
CC(1/2)	0.990	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	PEG/Ion Screen-G8: 2% v/v Tacsimate, pH 5.0, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 16% w/v PEG 3350