248D
CRYSTAL STRUCTURES OF AN A-FORM DUPLEX WITH SINGLE-ADENOSINE BULGES AND A CONFORMATIONAL BASIS FOR SITE SPECIFIC RNA SELF-CLEAVAGE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-09 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 25.890, 33.970, 57.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.830 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| R-free | 0.23600 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 3.280 |
| Data reduction software | R-AXIS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.780 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.081 |
| Number of reflections | 2264 |
| Completeness [%] | 94.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.4 | pH 7.40, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | oligonucleotide | 0.5 (mM) | ||
| 2 | 1 | sodium cacodylate | 10 (mM) | ||
| 3 | 1 | spermine tetrahydrochloride | 33 (mM) | ||
| 4 | 1 | magnesium chloride | 2 (mM) | ||
| 5 | 1 | MPD | 40 (%) |
