23VJ

Crystal structure of the MafR protein from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	63.179, 102.472, 90.755
Unit cell angles	90.00, 96.76, 90.00

Refinement procedure

Resolution	29.470 - 2.300
R-factor	0.2133
Rwork	0.211
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.788
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	51247	2585
<I/σ(I)>	14.8	0.9
Completeness [%]	99.8	100
Redundancy	3.3	3.2
CC(1/2)	0.996	0.509

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	ammonium sulfate, sodium citrate