23TZ

Crystal structure of cleaved DL-endopeptidase CwlO from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-06
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	58.548, 58.548, 111.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.810 - 2.100
R-factor	0.1986
Rwork	0.198
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.819
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.400	2.230
High resolution limit [Å]	2.100	2.100
Number of reflections	11908	1851
<I/σ(I)>	19.52
Completeness [%]	99.9
Redundancy	10.67
CC(1/2)	0.999	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) monohydrate, pH 6.5, 12% (w/v) polyethylene glycol 20,000