23TM

Structure of the 328-692 fragment of FlhA (A489E) form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	I 41
Unit cell lengths	215.970, 215.970, 64.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.990 - 3.280
R-factor	0.2185
Rwork	0.216
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a5i
RMSD bond length	0.003
RMSD bond angle	0.645
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.990	3.540
High resolution limit [Å]	3.280	3.280
Rmerge	0.075	0.458
Number of reflections	23119	4751
<I/σ(I)>	12	3.3
Completeness [%]	99.8
Redundancy	3.9
CC(1/2)	0.998	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% PEG3000, 0.1 M HEPES-NaOH, 0.2 M NaCl. Protein conc.: 10 mg/mL