23QH

Structure of importin alpha bound to the Psittacine Adenovirus B Fiber 1 protein nuclear localization signal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	296
Detector technology	PIXEL
Collection date	2025-08-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	P 21 21 21
Unit cell lengths	78.732, 89.780, 99.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.100
R-factor	0.1978
Rwork	0.197
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.499
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.930	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	41999	3402
<I/σ(I)>	16.4
Completeness [%]	100.0
Redundancy	7.6
CC(1/2)	0.999	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.6 M sodium citrate, 0.1 M HEPES