23JZ

pre-miR-6074 internal loop in complex with amiloride (Form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 31 2 1
Unit cell lengths	65.102, 65.102, 69.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.610 - 2.900
R-factor	0.1939
Rwork	0.191
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.997
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.610	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.120	0.382
Number of reflections	3993	287
<I/σ(I)>	11.57
Completeness [%]	99.8
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Potassium chloride, Calcium chloride dihydrate, Sodium cacodylate trihydrate, Polyethylene glycol 4000