23JY

pre-miR-6074 internal loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 61
Unit cell lengths	66.662, 66.662, 53.348
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.870 - 2.800
R-factor	0.1962
Rwork	0.191
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.469
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.870	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.124	0.890
Number of reflections	3137	190
<I/σ(I)>	13.31
Completeness [%]	92.6
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Sodium chloride, Spermine, MPD, MOPS