23EI

Artificial Copper-binding dimeric protein 1 (Cu(II)DP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	51.980, 63.680, 72.430
Unit cell angles	89.88, 89.86, 66.61

Refinement procedure

Resolution	28.870 - 2.240
R-factor	0.2376
Rwork	0.235
R-free	0.29390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.525
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.870	2.320
High resolution limit [Å]	2.240	2.240
Number of reflections	39097	3466
<I/σ(I)>	7.99
Completeness [%]	95.3
Redundancy	3.45
CC(1/2)	0.982	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M Tris-HCl (pH 8.5) buffer with 0.2 M NaCl and 17 % (w/v) PEG 3350